Original Article CD133, Trop-2 and α2β1 integrin surface receptors as markers of putative human prostate cancer stem cells
Marco Trerotola, Swati Rathore, Hira Lal Goel, Jing Li, Saverio Alberti, Mauro Piantelli, Dave Adams, Zhong Jiang, Lucia R. Languino
Department of Cancer Biology, Prostate Cancer Discovery and Development Program, University of Massachusetts Medical School, Worcester, MA 01605, USA; Department of Biology/Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, USA; Unit of Cancer Pathology, Department of Oncology and Neurosciences and CeSI, Foundation University “G. d’Annunzio”, Chieti Scalo, Italy; Department of Pathology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Received March 3, 2010; accepted March 10, 2010, available online March 15, 2010
Abstract: Cancer stem cells (CSCs) play a key role in initiation and development of cancer and are attractive targets for therapy. The identification of CSC surface receptors to be used as therapeutic targets in vivo remains a difficult task. In this study, we assessed the expression pattern of three surface receptors: CD133, Trop-2 and α2β1 integrin in human prostate cancer in order to identify CSC-niches. CD133 was found to be expressed in small clusters of cells localized in focal areas of benign as well as malignant lesions, suggesting this protein as a bona fide marker of the stem compartment. Trop-2 was localized in both basal and luminal layers of benign glands and was highly expressed in malignant lesions. Moreover, isolated cells in benign and malignant areas were found to co-express both CD133 and Trop-2. α2β1 integrin was expressed in the prostatic epithelium as well as in the surrounding stroma, limiting its utility as a marker of CSCs. In summary, we demonstrate that the combination of CD133 and Trop-2 is useful to mark putative CSC-containing compartments in human prostate. (AJTR1003002).
Address all correspondence to: Department of Cancer Biology Prostate Cancer Discovery and Development Program University of Massachusetts Medical School Worcester, MA 01605, USA E-mail: email@example.com