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Am J Translational Res 2011;3(2):159-165
Original Article
Validation of a novel, physiologic model of experimental acute
pancreatitis in the mouse
Kathryn M. Ziegler, Terence E. Wade, Sue Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas J. Zyromski
Department of Surgery, Indiana University, Indianapolis, IN, USA
Received November 23, 2010; Accepted December 20, 2010; Epub December 23, 2010; Published January 1,
2011
Abstract: Background: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We
sought to validate a recently reported murine model of acute pancreatitis that more closely represents the
physiology of human biliary pancreatitis. Methods: Mice (C57BL/6J and CF-1) underwent infusion of 50μl of 5%
sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours
later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue
concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA.
Results: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice
infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory
mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. Discussion: Retrograde
pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely
representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to
elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.(AJTR1011005).
Keywords: Acute pancreatitis, animal model, murine, sodium taurocholate, retrograde injection
Full Text PDF
Address all correspondence to:
Nicholas J Zyromski, MD
Assistant Professor,
Department of Surgery
Indiana University School of Medicine
535 Barnhill Dr. RT 130
Indianapolis, IN 46202
Ph 317-274-5012
Fax 317-274-4554
Email: nzyromsk@iupui.edu
Original Article
Validation of a novel, physiologic model of experimental acute
pancreatitis in the mouse
Kathryn M. Ziegler, Terence E. Wade, Sue Wang, Deborah A. Swartz-Basile, Henry A. Pitt, Nicholas J. Zyromski
Department of Surgery, Indiana University, Indianapolis, IN, USA
Received November 23, 2010; Accepted December 20, 2010; Epub December 23, 2010; Published January 1,
2011
Abstract: Background: Many experimental models of acute pancreatitis suffer from lack of clinical relevance. We
sought to validate a recently reported murine model of acute pancreatitis that more closely represents the
physiology of human biliary pancreatitis. Methods: Mice (C57BL/6J and CF-1) underwent infusion of 50μl of 5%
sodium taurocholate (NaT) or 50μl of normal saline (NaCl) directly into the pancreatic duct. Twenty-four hours
later, pancreatitis severity was graded histologically by three independent observers, and pancreatic tissue
concentration of interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) were determined by ELISA.
Results: Twenty four hours after retrograde injection, the total pancreatitis score was significantly greater in mice
infused with NaT than in those infused with NaCl (6.3 ± 1.2 vs. 1.2 ± 0.4, p<0.05). In addition, the inflammatory
mediators IL-6 and MCP-1 were increased in the NaT group relative to the NaCl group. Discussion: Retrograde
pancreatic duct infusion of sodium taurocholate induces acute pancreatitis in the mouse. This model is likely
representative of human biliary pancreatitis pathophysiology, and therefore provides a powerful tool with which to
elucidate basic mechanisms underlying the pathogenesis of acute pancreatitis.(AJTR1011005).
Keywords: Acute pancreatitis, animal model, murine, sodium taurocholate, retrograde injection
Full Text PDF
Address all correspondence to:
Nicholas J Zyromski, MD
Assistant Professor,
Department of Surgery
Indiana University School of Medicine
535 Barnhill Dr. RT 130
Indianapolis, IN 46202
Ph 317-274-5012
Fax 317-274-4554
Email: nzyromsk@iupui.edu
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